Comparison of Cepheid® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1
Identifieur interne : 000E52 ( Main/Exploration ); précédent : 000E51; suivant : 000E53Comparison of Cepheid® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1
Auteurs : Ali A. Rabaan [Arabie saoudite] ; Ali M. Bazzi [Arabie saoudite] ; Sana A. Alshaikh [Arabie saoudite]Source :
- Diagnostic Microbiology and Infectious Disease [ 0732-8893 ] ; 2017.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Arabie saoudite, Enfant, Enfant d'âge préscolaire, Femelle, Grippe humaine (diagnostic), Humains, Jeune adulte, Mâle, Nourrisson, Nouveau-né, Réaction de polymérisation en chaîne (), Sensibilité et spécificité, Sous-type H1N1 du virus de la grippe A (isolement et purification), Sujet âgé, Sujet âgé de 80 ans ou plus, Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Grippe humaine.
- isolement et purification : Sous-type H1N1 du virus de la grippe A.
- Adolescent, Adulte, Adulte d'âge moyen, Arabie saoudite, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mâle, Nourrisson, Nouveau-né, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Sujet âgé, Sujet âgé de 80 ans ou plus, Techniques de diagnostic moléculaire.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Female, Humans, Infant, Infant, Newborn, Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza, Human (diagnosis), Male, Middle Aged, Molecular Diagnostic Techniques (methods), Polymerase Chain Reaction (methods), Saudi Arabia, Sensitivity and Specificity, Young Adult.
- MESH :
- geographic : Saudi Arabia.
- diagnosis : Influenza, Human.
- isolation & purification : Influenza A Virus, H1N1 Subtype.
- methods : Molecular Diagnostic Techniques, Polymerase Chain Reaction.
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Female, Humans, Infant, Infant, Newborn, Male, Middle Aged, Sensitivity and Specificity, Young Adult.
Abstract
To compare two influenza polymerase chain reaction (PCR) methods.
A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid® Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay.
There was 92.12% overall agreement between the two methods. Specificity of the Cepheid® Xpert Flu was 95.8% for H1N1 and 94.4% for total influenza A. Cepheid® Xpert Flu sensitivity for influenza A was 100% for younger patients (0–19-year age group) but significantly lower both for older patients (68.2% for 60–79-year and 50% for ≥80-year age groups) and overall for males compared to females (72.6% and 94.0%, respectively).
Specificity of the Cepheid® Xpert Flu test was high; however, sensitivity for total influenza A was lower particularly in males and older patients.
Url:
DOI: 10.1016/j.diagmicrobio.2017.12.010
PubMed: 29329754
PubMed Central: 7132748
Affiliations:
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Bazzi</name><affiliation wicri:level="1"><nlm:aff id="af0010">Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311</wicri:regionArea><wicri:noRegion>Dhahran 31311</wicri:noRegion></affiliation></author><author><name sortKey="Alshaikh, Sana A" sort="Alshaikh, Sana A" uniqKey="Alshaikh S" first="Sana A." last="Alshaikh">Sana A. 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Rabaan</name><affiliation wicri:level="1"><nlm:aff id="af0005">Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311</wicri:regionArea><wicri:noRegion>Dhahran 31311</wicri:noRegion></affiliation></author><author><name sortKey="Bazzi, Ali M" sort="Bazzi, Ali M" uniqKey="Bazzi A" first="Ali M." last="Bazzi">Ali M. Bazzi</name><affiliation wicri:level="1"><nlm:aff id="af0010">Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311</wicri:regionArea><wicri:noRegion>Dhahran 31311</wicri:noRegion></affiliation></author><author><name sortKey="Alshaikh, Sana A" sort="Alshaikh, Sana A" uniqKey="Alshaikh S" first="Sana A." last="Alshaikh">Sana A. Alshaikh</name><affiliation wicri:level="1"><nlm:aff id="af0015">Molecular Diagnostic laboratory, Dammam Regional Laboratory, Ministry of Health, Dammam, 31675, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Molecular Diagnostic laboratory, Dammam Regional Laboratory, Ministry of Health, Dammam, 31675</wicri:regionArea><wicri:noRegion>31675</wicri:noRegion></affiliation></author></analytic><series><title level="j">Diagnostic Microbiology and Infectious Disease</title><idno type="ISSN">0732-8893</idno><idno type="eISSN">1879-0070</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term><term>Influenza, Human (diagnosis)</term><term>Male</term><term>Middle Aged</term><term>Molecular Diagnostic Techniques (methods)</term><term>Polymerase Chain Reaction (methods)</term><term>Saudi Arabia</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Arabie saoudite</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Grippe humaine (diagnostic)</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Nourrisson</term><term>Nouveau-né</term><term>Réaction de polymérisation en chaîne ()</term><term>Sensibilité et spécificité</term><term>Sous-type H1N1 du virus de la grippe A (isolement et purification)</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Techniques de diagnostic moléculaire ()</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Male</term><term>Middle Aged</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Arabie saoudite</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Nourrisson</term><term>Nouveau-né</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Techniques de diagnostic moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Objective</title><p>To compare two influenza polymerase chain reaction (PCR) methods.</p></sec><sec><title>Methods</title><p>A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid® Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay.</p></sec><sec><title>Results</title><p>There was 92.12% overall agreement between the two methods. Specificity of the Cepheid® Xpert Flu was 95.8% for H1N1 and 94.4% for total influenza A. Cepheid® Xpert Flu sensitivity for influenza A was 100% for younger patients (0–19-year age group) but significantly lower both for older patients (68.2% for 60–79-year and 50% for ≥80-year age groups) and overall for males compared to females (72.6% and 94.0%, respectively).</p></sec><sec><title>Conclusions</title><p>Specificity of the Cepheid® Xpert Flu test was high; however, sensitivity for total influenza A was lower particularly in males and older patients.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Al Tawfiq, J" uniqKey="Al Tawfiq J">J. Al-Tawfiq</name></author><author><name sortKey="Abed, M" uniqKey="Abed M">M. Abed</name></author><author><name sortKey="Saadeh, B M" uniqKey="Saadeh B">B.M. Saadeh</name></author><author><name sortKey="Ghandour, J" uniqKey="Ghandour J">J. 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Panning</name></author><author><name sortKey="Kaul, K L" uniqKey="Kaul K">K.L. Kaul</name></author><author><name sortKey="Mangold, K A" uniqKey="Mangold K">K.A. Mangold</name></author><author><name sortKey="Revell, P A" uniqKey="Revell P">P.A. Revell</name></author><author><name sortKey="Luna, R A" uniqKey="Luna R">R.A. Luna</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="World Health Organization Who" uniqKey="World Health Organization Who">World Health Organization (WHO)</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Arabie saoudite</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Rabaan, Ali A" sort="Rabaan, Ali A" uniqKey="Rabaan A" first="Ali A." last="Rabaan">Ali A. Rabaan</name></noRegion><name sortKey="Alshaikh, Sana A" sort="Alshaikh, Sana A" uniqKey="Alshaikh S" first="Sana A." last="Alshaikh">Sana A. Alshaikh</name><name sortKey="Bazzi, Ali M" sort="Bazzi, Ali M" uniqKey="Bazzi A" first="Ali M." last="Bazzi">Ali M. Bazzi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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